Demonstrations of the MLAO method in microscopy imaging applications. (IMAGE)

Light Publishing Center, Changchun Institute of Optics, Fine Mechanics And Physics, CAS

Demonstrations of the MLAO method in microscopy imaging applications.

Caption

(a) MLAO was embedded in the microscope control to improve imaging quality by modulate the illumination light. Right-hand side showed an example of one operation cycle using MLAO. After using MLAO with two sample exposures, the imaging quality was largely improved as the image labelled as corrected was much brighter and sharper than the image labelled as initial. (b) MLAO was used in three-photon intravital deep tissue imaging of a mouse brain. After MLAO, the cells were brighter, sharper and more recognisable. (c) MLAO was used in super-resolution imaging of Drosophila larval neuromuscular junctions. After MLAO, the image was much sharper with a lot more fine features resolved and hexagonal artefacts removed.

Credit

by Qi Hu, Martin Hailstone, Jingyu Wang, Matthew Wincott, Danail Stoychev, Huriye Atilgan, Dalia Gala, Tai Chaiamarit, Richard M. Parton, Jacopo Antonello, Adam M. Packer, Ilan Davis & Martin J. Booth

Usage Restrictions

Credit must be given to the creator.

License

CC BY

Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.