Figure 1. Combination of site-directed photo-crosslinking and AllocLys-OH-mediated site-directed cleavages (IMAGE)

Tokyo Medical and Dental University

Figure 1. Combination of site-directed photo-crosslinking and AllocLys-OH-mediated site-directed cleavages

Caption

The concept of the present method is shown schematically. The crosslinker (pBpa, red circle) or the cleavage site (AllocLys-OH, blue circle) is introduced to Protein 1 or Protein 2, respectively. AllocLys-OH, the a-hydroxy form of AllocLys, forms an alkaline-labile ester bond within the main chain of a protein. After Proteins 1 and 2 are crosslinked, the crosslinked product is cleaved by an alkaline treatment. If the product retains the tag, the crosslinked residue is considered to be closer to the tagged end than the cleavage site in Protein 2 (upper). If the product loses the tag, then the crosslinked residue may be more distal from the tag than the cleavage site (lower).

Credit

Department of Biochemistry, TMDU

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