BioDesign Research Manuscript Template Page 12 of 35with bacteriophage φ80 was transformed with a Tet repressor expression plasmid (pKLC83) (IMAGE)
Caption
(a) Schematic diagram of the construction of a φ80 phage capsid containing T1-spanin gene. T1-spanin exhibits toxicity toward synthetic bacterium 594; therefore, its expression was suppressed using a Tet repressor-expressing plasmid (pKLC83). Due to the presence of a packaging sequence, the T1-spanin plasmid was encapsulated within a phage capsid. (b) The synthesized phage capsid (a) was used to infect Escherichia coli MC1061. Within bacterial cells lacking Tet repressor expression, T1-spanin was expressed. (c) E. coliMC1061 were infected with phage capsids containing either the T1-spanin-encoding plasmid or a plasmid lacking T1-spanin (control). The infected bacteria were plated on LB agar plates, and the number of colony-forming units (CFUs) were counted. Abbreviation: LB, Luria-Bertani. Assays were performed in triplicate. The bars show the means of the three spot test results, and the error bars show the standard deviations.
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BioDesign Research
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