Workflow of a typical base editor screening experiment. (IMAGE)

Science China Press

Workflow of a typical base editor screening experiment.

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A library with single guide RNA (sgRNA) is packed into lentiviral particles and transduced into editor-expressing cells. The sgRNA-transduced cells are selected to generate mutant cells. Mutant cells are treated and untreated. DNA is extracted, and sgRNA is amplified via polymerase chain reaction (PCR). Screening is conducted via deep sequencing before bioinformatics analysis (By Figdraw). ABE: adenine base editor; CBE: cytosine base editor; NGS: next-generation sequencing; SNV: single nucleotide variant; VUS: variants of uncertain significance.

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