An overview of CRISPR-Cas9-mediated imaging of genomic loci in cells (IMAGE)
Caption
This includes the labeling of the dCas9 protein, engineering of sgRNA, and the use of dCas9 orthologs from different bacterial species. CRISPR, clustered regularly interspaced short palindromic repeats; crRNA, CRISPR RNA; dCas9, dead Cas9; PUF, Pumilio and fem-3 binding factor; sgRNA, single-guide RNA; tracrRNA, transactivating RNA.
Credit
Geik Yong Ang, Choo Yee Yu
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License
CC BY-NC