Phenotypic characterization of flower buds, open flowers, and silique development in WT and four mutants. (IMAGE)
Caption
Phenotypic characterization of flower buds, open flowers, and silique development in WT and four mutants. A The growth and flowering of the WT and four mutants. Blue arrows indicate normally developed flower buds and opening flowers, while white arrows highlight abnormally developed flower buds and flowers. Scale bar = 5 cm. B Flower buds, buds without sepals and petals, and anthers in flower buds before flowering. Scale bar = 0.5 cm; dashed box scale bar = 0.25 cm. C Open flowers without sepals and petals, and anthers in fully opened flowers. Scale bar = 0.5 cm; dashed box scale bar = 0.25 cm. D Siliques at 12 DAP of WT and the four mutants. Scale bar = 2 cm. E, F Paraffin sections of transverse anther in flower buds 1–2 days before flowering in WT (E) and DM (L1) (F). Epidermis (ep), endothecium (en), stomium (st), connective (c), vascular bundle (v), and pollen grains (p). Scale bar = 1 mm. G Pollen viability of WT, L1, L2, L3, and L4 assessed after 5 h of room temperature staining with Alexander’s staining solution. Values in the bars represent means of three biological replicates, and error bars indicate standard deviations. Different lowercase letters above the bars indicate statistical significance at P < 0.05 based on Student’s t-test.
Credit
Horticulture Research
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