Correlation analysis between the expression of MCT4 in hepatocytes and NAFLD. (IMAGE)
Caption
(A, B) The scatter plots for Mct4 TPM of Normal (n = 31) and NAFLD (n = 112) in the GSE162694 dataset (A), and of NAFL (n = 51) and NASH (n = 47) in the GSE167523 dataset (B). Unpaired, two-sided Mann–Whitney U test P-values are depicted in the plots, and the significant P-value cutoff was set at 0.05. The plots show the medians (black line), standard deviation, and P-values. (C) The scatter plots for Mct4 TPM in GSE174478 (n = 94) in which patients were stratified by NAFLD activity score (NAS, left) or fibrosis score (F, right). P-values were obtained via a nonparametric two-stage Benjamini, Krieger, & Yekutieli false discovery rate (FDR) procedure. The mean expression (black line), standard deviation, and P-values are shown. (D) The immunohistochemical staining of protein levels of MCT4 in the livers of C57BL/6J mice fed with chow diet or high-fat diet (HFD). Representative positive stains are indicated with black arrows (100 × and 400 ×). (E) The immunohistochemical staining of MCT4 expression in hepatocellular carcinoma patients with NAFLD (HCC group) and adjacent non-tumor tissues without NAFLD (Para-Ca group). Representative positive stains are indicated with black arrows (100 × and 400 ×). Each assay condition was done in triplicate, and representative images were shown. (F) The expression of Mct4 in oleic acid (OA)-induced lipid accumulation in hepatocytes. Subconfluent iHPx cells were stimulated with 0.05 mM OA (methanol as a vehicle control), total RNA was isolated at 24 h and subjected to touchdown-quantitative PCR analysis of Mct4 expression. ∗∗P < 0.01, OA versus Methanol. MCT4, monocarboxylate transporter 4; NAFLD, non-alcoholic fatty liver disease; TPM, transcript per million; NAFL, non-alcoholic fatty liver; NASH, non-alcoholic steatohepatitis.
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Genes & Diseases
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