Sex-specific longitudinal reversal of aging in old frail mice (IMAGE)
Caption
Figure 4. Acute effects of OT+A5i on de novo systemic proteomes of males and females. (A) Schematics of the experimental approach. (B) Uniform Manifold Approximation and Projection (UMAP) plots of the profiles of de-novo synthesized systemically present proteins of young control, old control, and old OT+A5i mice, males and females. AHA and ANL data are merged. (C) Sex-annotated comparison of the mean SDs of the Levene’s test passed proteins (p < 0.05) with lower noise in young control vehicle mice compared to old control vehicle mice and in old mice administered with OT+A5i vs. vehicle control. In the shown line graphs, SDs of all groups are normalized by the SDs of the old vehicle control and presented on a log scale. (D) cirFunMap visualization of KEGG enriched pathways of de-novo synthesized systemic proteomes after OT+A5i treatment. (E) cirFunMap visualization of KEGG enriched pathways of the de-novo synthesized proteins that are noisier in the old as compared to young mice and become youthfully normalized by OT+A5i in old males and females. n = 4 each young and old MetRSL274G vehicle control, n = 4 each young and old C57.B6 vehicle control, n = 4 old OT+A5i C57.B6.
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Copyright: © 2025 Kato et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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