The impact of long-term social housing on biconditional association task performance and neuron ensembles in the anterior cingulate cortex and the hippocampal CA3 region of aged rats (IMAGE)
Caption
Figure 1. Experimental methods. (A) Timeline of BAT training and test day. Green circles represent correct choice; black circles represent incorrect choice. Object placement over the left and right food well was randomized and counterbalanced across trials. Test day epochs were counterbalanced across subjects so that half of the animals were tested on the alternation task (ALT) first and the other half were tested on the biconditional association task (BAT) first. (B) Brain regions imaged (ACC: yellow, CA3: pink). Black dashed line and blue solid line squares indicate deep and superficial areas of the ACC and distal and proximal areas of the CA3 respectively. (C) Immediate early gene cellular distribution for neurons that were active during baseline, during epoch 1 only, during epoch 2 only, during both epochs, and during both epochs and baseline. Red dots signify Arc expression and green dots signify Homer1a expression. (D) Representative microscopic image of subcellular distribution of Arc (red) and Homer1a (green) within neurons where the nuclei were counterstained with DAPI (blue). Scale bar is 50 μm.
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Copyright: © 2025 Dankert et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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