The acoustofluidic platform for capture and detection of sEVs via specific surface markers (IMAGE)
Caption
(A) The acoustofluidic chip comprises a channel containing sharp-edge microstructures that can be acoustically activated with an acoustic buzzer. (B) In the absence of acoustic activation, no fluorescence signal is detected as sEVs pass through the channel in the detection region. (C) When the chip is acoustically activated, sEVs containing specific surface markers are concentrated at the tips of the sharp-edge microstructures and detectable by fluorescence microscopy. (D) Experimental demonstration of on-chip detection of sEVs based on their surface proteins. A fluorescent signal is observed when the target protein is present above the detection threshold, while no signal appears if the protein is absent. CD63 is used as a control biomarker due to its common presence on sEVs. Scale bar, 100 μm.
Credit
Jessica F. Liu, Duke University School of Medicine.
Usage Restrictions
News organizations may use or redistribute this image, with proper attribution, as part of news coverage of this paper only.
License
Licensed content