Flowchart illustrating the experimental design. (IMAGE)
Caption
The overarching experimental design is depicted in Figure . On day 1, each cell type was seeded on uniquely colored cell carriers (Huh7 on white, THP1 on red, and LX2 on blue). On day 2, the Huh7 cells underwent continued culturing, whereas THP1 cells were stimulated with lipopolysaccharide (LPS) and LX2 cells were treated with transforming growth factor-β1 (TGF-β1) for 24 h. On day 3, the 3 different MASLD models were established based on the cocultured cell types: steatosis (hepatocytes only), MASH (hepatocytes and macrophages in a 4:1 ratio), and fibrosis (hepatocytes, macrophages, and HSCs in an 8:2:1 ratio) with reference to previous studies . After 24 h of coculturing, the different cell types were harvested individually for further analysis.
Credit
Xiao Lab @GU.
Usage Restrictions
News organizations may use or redistribute this image, with proper attribution, as part of news coverage of this paper only.
License
Original content