CD4 T Cells (IMAGE)
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a, Experimental setup: splenocytes were collected from young CD45.1 mice; then, 20 million of these cells were injected into either young (2–3-month-old; n = 6) or old (22–24-month-old; n = 4) CD45.2 mice, followed 30 days later by the collection of spleens for analysis. b, Stacked bar chart showing a bulk flow cytometry analysis of transferred (CD45.1+CD3+CD4+CD8−) T cells in young and old mice, showing the proportions of CD62L+CD44− cells (naive cells, blue), CD44+PD1− (effector memory cells, green), Eomes+CCL5+ (CD4-Eomes, red), CD44+PD1+ (orange) and FOXP3+ cells (Treg, gray). c, Representative flow cytometry plots illustrating the proportions of CD4-Eomes (Eomes+CCL5+) out of CD4 T cells (CD3+CD4+CD8−) among transferred cells (CD45.1+) in young or old CD45.2 mice. d, Quantitative analysis of CD4-Eomes cells among transferred cells in young (n = 6) or old (n = 4) WT mice (CD45.2). e, Quantitative analysis of CD62L+CD44−, FOXP3+, CD44+PD1− and CD44+PD1+ CD4 T cells among transferred cells in young or old WT mice (CD45.2). f, Graph showing the comparison of CD4-Eomes cell percentages out of CD4+ cells between CD45.1 (young-transferred cells) and CD45.2 (old endogenous cells) in the old group (n = 4). The bars represent the mean ± s.e.m. from two independent experiments. Data were analyzed using a two-tailed Student’s t-test (unpaired in d,e and paired in f). Exact P values are presented in the figures.
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