Overview of the tobacco rattle virus-mediated genome editing system in tobacco plants (IMAGE)
Caption
Overview of the tobacco rattle virus-mediated genome editing system in tobacco plants. A 20-nucleotide target sequence is cloned before the sgRNA scaffold under the PEBV promoter in the pTRV2-PEBV plasmid. Agrobacterium (GV3101) cultures harboring both pTRV1 and the engineered pTRV2-PEBV vectors are co-infiltrated into the cotyledons of Cas9-expressing tobacco plants. After agro-infiltration, their systemic leaves are analyzed for the presence of indel mutations at the targeted site. Eventually, tobacco mutants are isolated by screening the harvested seeds, eliminating the need for additional tissue culture.
Image link: https://academic.oup.com/view-large/figure/507805838/uhae364f1.tif
Credit
Horticulture Research
Usage Restrictions
Credit must be given to the creator.
License
CC BY