The characteristics of CRISPR/Cas9-edited Sldnaj lines after TSWV inoculation. (IMAGE)
Caption
The characteristics of CRISPR/Cas9-edited Sldnaj lines after TSWV inoculation. (A) Diagrammatic sketch of SlDnaJ targeting the two single-guide RNAs (sgRNAs). (B) Sequences of the edited regions of the CRISPR/Cas9-edited Sldnaj mutants (Sldnaj-cr1–5 and Sldnaj-cr17–3) and wild-type (WT). The two sgRNA target sites are indicated in Target 1 and Target 2 and the altered sequences in the edited line are indicated in blue. The Sldnaj-cr alleles were confirmed by sequencing of the PCR products from two T1 plants in the M82 background. (C) The leaf symptom phenotypes of the WT, Sldnaj-cr1–5, and Sldnaj-cr17–3 lines at 0, 14, 28, and 35 days postinoculation (dpi). (D) DAB and NBT staining of Sldnaj-cr lines at 7 dpi. (E) RT-qPCR detection of the expression of TSWV-cp in Sldnaj-cr lines at 0, 7, 14, 28, and 35 dpi. (F) RT-qPCR detection of the Sldnaj transcription levels in the Sldnaj-cr lines at 0, 7, 14, 28, and 35 dpi. (G) The ratio of DAB and NBT staining areas of the Sldnaj-cr lines. The error bars represent the standard deviations three biological replicates. Two asterisks and three asterisks indicate significant differences at P < 0.01 and P < 0.001, respectively.
Image link: https://academic.oup.com/view-large/figure/508297153/uhaf019f6.tif
Credit
Horticulture Research
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