Figure 1 (IMAGE)
Caption
Confocal laser scanning microscopy (CLSM) images of Aegilops cylindrica leaves infected with IPO323 (incompatible) and Zt469 (compatible) Zymoseptoria tritici isolates. Microscopic images from CLSM analysis using maximum projections of image z-stacks. Cell nuclei and plant tissue are visible in purple, and fungal hyphae are in green. Except for the 4 days postinfection (dpi) sample of the incompatible interaction, only fungal hyphae growing within the plant tissue are shown. Letters indicate which infection stage is represented by the images. Time points listed in purple were chosen for RNA sequencing analysis. A, The four infection stages (A to D) can be observed during compatible interaction, representing all stages from early biotrophy to late necrotrophy. B, Incompatible interaction samples show only fungal proliferation on the surface and penetration through stomata (stages 0 and A).
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