Overview of the manufacturing process of personalized neoantigen-based DC vaccines. (IMAGE)
Caption
Overview of the manufacturing process of personalized neoantigen-based DC vaccines. A typical basic process of manufacturing neoantigen-based DC vaccines by using monocyte-derived dendritic cells is depicted. First, patients’ peripheral blood mononuclear cells collected via leukapheresis are isolated and purified for collection of monocytes. Subsequently, monocytes are cultured and differentiated into immature DCs with the addition of a series of stimulatory cytokines. Subsequently, neoantigens (e.g., nucleic acid, peptides, tumor cells, or lysates) are loaded with immature DCs for further cultivation of mature DCs with the addition of a cocktail of stimulation factors. Finally, neoantigen-loaded DCs are collected for quality testing and ultimately used for vaccination in patients.
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Cancer Biology & Medicine
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