Enhanced Pst DC3000 resistance of SlATL2/SlCSN5a-silenced tomato plants. (IMAGE)
Caption
Enhanced Pst DC3000 resistance of SlATL2/SlCSN5a-silenced tomato plants. (A) Representative symptoms and (B) bacterial populations in leaves 3 dpi with Pst DC3000 in VIGS plants targeting the indicated genes. (C) Expression profiling of immune-related genes. Leaves were harvested at 24 hpi, and gene expression was analyzed by qPCR using SlActin as the reference gene. (D) Salicylic acid (SA) contents. (E-F) Accumulation of H2O2. Bars = 5 mm. DAB staining intensity, as determined with ImageJ software. Data were shown as means ± SD; n = 3 (B, C, D, F). Statistically significant differences are indicated by asterisks (*P < 0.05, **P < 0.01, and ***P < 0.001, Student’s t-test) (C). Statistically significant differences are denoted by different letters, as determined by two-way ANOVA followed by Tukey’s test (P < 0.05) (B, D, F). (F) Proposed working model of SlATL2 function in tomato immunity. Upon infection with Pst DC3000, in WT plants, SlATL2 induces the degradation of SlCSN5a, suppressing the SA signaling pathway and ROS production, leading to susceptibility to Pst DC3000. In contrast, the slatl2 mutant accumulates SlCSN5a, which activates the SA pathway and increases ROS, conferring resistance to the pathogen.
Credit
Horticulture Research
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