Workflow of syngas biomethanation under increasing hydrogen ratios. (IMAGE)
Caption
Workflow of syngas biomethanation under increasing hydrogen ratios. This schematic illustrates the experimental workflow used to evaluate how hydrogen enrichment affects syngas-converting microbiomes. Cultures were initially supplied with baseline syngas (69% H₂, 16% CO₂, 15% CO), followed by stepwise hydrogen increases to 77% and 84%. Samples collected across stages were analyzed using metagenomics, metatranscriptomics, and virome profiling to track changes in microbial composition, viral populations, and metabolic pathways. The approach enabled quantitative comparison of community abundance and activity, revealing metabolic reprogramming and defense activation under hydrogen-rich conditions.
Credit
Environmental Science and Ecotechnology
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