Figure | PSF decoupling and deconvolution. (IMAGE)
Caption
Figure | PSF decoupling and deconvolution. a Schematic diagram of CFM, the system response can be altered through the aperture modulation of the equivalent pupil to optimize imaging performance; b principle and workflow of PSF decoupling, by using a fluorescent sample as a modulator and taking its wide-field deconvolution result as a sample prior, the system PSF can be computationally optimized from the detection signal and finally applied to the deconvolution of any observed fluorescent samples; c MIP of z-stack of a potato tuber sample; d, e local enlarged views of x-y slices at different ROIs, comparing the raw image and deconvolution results using BD, mPSF, and cPSF, respectively; f cross-sectional intensity distribution curves related to regions marked by white dashed lines in (e); 3D profiles along with central y-z and marginal x-y slices of g mPSF and h cPSF. Scale bars, 100 μm (c), 20 μm (d), 15 μm (e, g, h).
Credit
Zewei Cai et al.
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Credit must be given to the creator.
License
CC BY