Cell Counting Imager (IMAGE)
Caption
Cell Counting Imager (CCI), a custom device that measures cell concentrations in 8 samples at once, to calculate the appropriate volumes of cell suspension needed to seed a specified number of cells in each destination well. The imager is an inverted darkfield microscope featuring a custom flow cell with eight chambers and a motorized linear stage that positions the optical system to image each chamber. The flow cell mounts on top of the CCI and has a row of ports that allow the multichannel pipette on the OT-2 robot to inject samples from one column of the source plate at a time.
Credit
Rafael Gomez-Sjoberg
Usage Restrictions
None
License
Original content