Figure 2. The production of 1-dodecanol from engineered E. coli BL21(DE3). (IMAGE)

Nanjing Agricultural University The Academy of Science

Figure 2. The production of 1-dodecanol from engineered E. coli BL21(DE3).

Caption

(A) A schematic diagram of 1-dodecanol biosynthetic pathway (Yunus et al., 2018). Heterologous enzymes, including Tes12, CAR, and Sfp, are labelled in red. The native pathway and enzyme, fatty acid synthesis (FAS) pathway and aldehyde reductase (AHR) enzyme, are labelled in blue (B) Plasmid maps and their regulatory architecture for GFP expression as a negative control and 1-dodecanol production (C) GC-FID chromatograms of (1) 1-dodecanol standard, solvent overlays sampled from (2) E. coli BL21(DE3) carrying pET-GFP and (3) E. coli BL21(DE3) carrying pET-Tes12-Sfp-CAR.

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