Figure 1. Construction of an efficient chondroitin sulfate A (CSA) cell factory in K. phaffii. (IMAGE)

Nanjing Agricultural University The Academy of Science

Figure 1. Construction of an efficient chondroitin sulfate A (CSA) cell factory in K. phaffii.

Caption

Three modules have been integrated into K. phaffii: chondroitin precursor synthesis, chondroitin 4-O-sulfotransferase (CHST11) protein engineering, and a 3′-adenosine 5′-phosphate (PAPS) regeneration system. Fru-6-P, fructose-6-phosphate; UDP-Glc, UDP-glucose; UDP-GlcA, UDP-glucuronate; UDP-GlcNAc, UDP-N-acetylglucosamine; UDP-GalNAc, UDP-N-acetylgalactosamine; PAP, 3'-phosphoadenosine-5'-phosphate; AMP, adenosine monophosphate; ADP, adenosine diphosphate; ATP, adenosine triphosphate; PAPS, 3'-phosphoadenosine-5'-phosphosulfate; TuaD, UDP-glucose 6-dehydrogenase; KfoA, UDP-N-acetylglucosamine-4-epimerase; KfoC, chondroitin polymerase; ATPS, ATP sulfurylase; APSK, APS kinase; PPK, polyphosphate kinase; BPNT, bisphosphate 3'-nucleotidase; APSST, adenine-sulfotransferase; SUL1/SUL2, sulfate transporters. A comprehensive list of abbreviations used in this manuscript is provided in Supplementary Table S1.

Credit

The authors

Usage Restrictions

Credit must be given to the creator.

License

CC BY

Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.