Establishment of a CRISPR/Cas9-based base-editing system and multigene knock-out process in Trichoderma koningiopsis. (IMAGE)

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Establishment of a CRISPR/Cas9-based base-editing system and multigene knock-out process in Trichoderma koningiopsis.

Caption

mini-SDD7 denotes the mini-SDD7 deaminase; nCas9 denotes Cas9 nickase (D10A); UGI denotes uracil glycosylase inhibitor; sgRNA-yak1, sgRNA-ace1, and sgRNA-pk1 denote the sgRNA expression cassettes targeting the yak1, ace1, and pk1 genes, respectively; AMA1 denotes the autonomous replication sequence.

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Mycology

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