Epigenetic dysregulation and biological function of PDX1 in prostate cancer (IMAGE)
Caption
Figure 5: Analysis of glucose-dependent PDX1 activity and metabolic markers in prostate cancer cells.
(A) Cell proliferation assay evaluating the effect of varying glucose concentrations (5 mM, 10 mM, 25 mM, and 50 mM; or no glucose- mock) in PC-3 cells stably expressing PDX1 overexpression or with stable shRNA PDX1 knockdown. Cells were counted at 24, 48, and 72 hrs post-treatment. Mock treated cells are set to 100%. (B) Relative qRT-PCR analysis of ESR2, IGF1R, and INSR in response to high glucose treatment compared to low glucose in PC3 cells with stable PDX1 expression or stable shRNA PDX1 knockdown compared to respective controls. (C) Relative qRT-PCR analysis of CXCR7, and TNFα in response to high glucose treatment compared to low glucose in PC3 with stable PDX1 expression or stable shRNA PDX1 knockdown compared to respective controls. (D) Relative qRT-PCR analysis of SNAI1, CDH2, and TWIST1 in response to high glucose treatment compared to low glucose in PC3 cells with stable PDX1 expression or stable shRNA PDX1 knockdown compared to respective controls. Controls are set to 1 for (B–D). Data shown are representative of three independent experiments.
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Copyright: © 2026 Adeyika et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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