Two-stage microfluidic enrichment of tumor cells and clusters. (IMAGE)
Caption
Two-stage microfluidic enrichment of tumor cells and clusters. The schematic shows how pleural effusion samples from lung cancer patients are processed through a cascaded inertial microfluidic device to enrich single MTCs and intact MTCCs. In the first stage, parallelized serpentine channels remove most background blood cells at high throughput. In the second stage, a slanted spiral sorter separates single tumor cells from larger clusters based on size-dependent inertial focusing. The enriched cells are then identified by immunofluorescence, supporting improved malignancy assessment and metastatic profiling.
Credit
Microsystems & Nanoengineering
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