Engineering the glycocalyx of NK-92MI cells via MGE and ST6Gal1-assisted CeGE. (IMAGE)
Caption
(a) Schematic of glycocalyx engineering-based incorporation of azide-bearing Sia onto surface glycans. (b) Quantification of the 1,6Pr2ManNAz-assisted MGE labeling of NK-92MI cells by flow cytometry. (c) Quantification of the ST6Gal1-assisted CeGE labeling of NK-92MI cells by flow cytometry. (d) Time-dependent MGE labeling of NK-92MI cells with 1,6Pr2ManNAz or Ac4ManNAz. Equal loading was confirmed by Coomassie brilliant blue staining (CBB). (e) Comparison of the labeling efficiency in MGE- and CeGE-treated NK-92MI cells. The error bars in (b) and (c) are expressed as the mean ± standard deviation (s.d.), n = 3. Ac4ManNAz: per-O-acetylated N-azdioacetylmannosamine; Neu5Az: N-azidoacetylneuraminic acid; CMP: cytidine monophosphate; Cy5: cyanine 5; GlcNAc: N-acetylglucosamine; NC: negative control; MFI: mean fluorescence intensity; a.u.: arbitrary unit.
Credit
Yuxin Li, Tao Gao et al.
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