Ectopic expression of CLCA4 induced reduced proliferation, invasion, motility, and stem cell-like properties of colorectal cancer (CRC) cells. (IMAGE)
Caption
(A) CLCA4 reduced CRC cell proliferation. The survival rates were assessed by the CCK8 assay. (B) CLCA4 inhibited CRC cell proliferation. The colony sizes are shown in the left panels, and the colony numbers are shown in the right panels. Unpaired two-tailed t-test (mean ± standard deviation). (C) The motility and invasion of CLCA4 overexpressing CRC cells and control cells were detected by transwell migration and Boyden chamber invasion assays. Unpaired two-tailed t-test (mean ± standard deviation). (D) The mRNA expression of Bmi-1, Oct4, ABCG2, E-cadherin, and vimentin in CLCA4-expressing and control CRC cells was detected by PCR. Unpaired two-tailed t-test (mean ± standard deviation). (E) The protein expression of CLCA4, Bmi-1, Oct4, ABCG2, E-cadherin, and vimentin in CLCA4-expressing and control CRC cells was assessed using Western blotting analysis. Lower panels: Quantification of protein expression ratio. (F) CLCA4 suppressed CRC cell tumorsphere formation. The tumorsphere sizes are shown in the left panels, and the tumorsphere numbers are shown in the right panels. Unpaired two-tailed t-test (mean ± standard deviation).
Credit
Fang Wei, Qi Zou, Qihui Sun, Tingting Jiang, Tian Cai, Xiaojia Li, Keping Xie
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