Workflow and Key Findings of Size-Dependent Nanoplastic Distribution in the Neonatal Mouse Brain (IMAGE)
Caption
Fluorescently labeled polystyrene nanoplastics were orally administered to neonatal mice, and tissues were collected 24 hours later for imaging. Following optical tissue clearing, whole-brain three-dimensional imaging was performed using light-sheet fluorescence microscopy. The analysis revealed that 50 nm polystyrene nanoplastics accumulated more extensively in the neonatal brain than larger 500 nm particles. Whole-brain visualization and regional analysis indicated higher fluorescence signals in the thalamus and brainstem than in other regions following exposure to 50 nm nanoplastics, highlighting a particle size-dependent pattern of biodistribution.
Credit
National Institute for Environmental Studies (NIES)
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CC BY-NC