Disruption of the insulin/IGF-1 signaling pathway in Caenorhabditis elegans dramatically increases male longevity and enhances reproductive health late in life (IMAGE)
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Figure 1. Ubiquitous and tissue-specific DAF-2 degradation extends male lifespan. (A) Kaplan-Meier curves showing survival of C. elegans hermaphrodites and males following ubiquitous DAF-2 degradation. Purple lines denote hermaphrodites and green lines denote males. Dashed lines denote the negative controls (TIR1-only on auxin) and solid lines denote DAF-degradation with 1mM auxin treatment. Each line represents at least two biological replicates with total n = 183–235. (B) Kaplan-Meier curves showing survival of C. elegans males aged on either individual or group plates. The gray line denotes the negative controls treated with ethanol and the green line denotes ubiquitous DAF-degradation with 1mM auxin treatment. The dashed lines denote single males and the solid lines denote grouped males. Each line represents three biological replicates with total n = 69–200. (C, D) Kaplan-Meier curves showing survival of C. elegans males following DAF-2 degradation in the (C) intestine and (D) germline. Gray lines denote the negative controls treated with ethanol and green lines denote DAF-degradation with 1mM auxin treatment. Each line represents at least two biological replicates with total n = 137–218. The black dashed lines denote the age at which 50% of the population has died. The asterisks denote p-values from a Cox Proportional Hazards model where ****p<.0001, ***p<.001. For additional information and the output of the CPH model, see Supplementary Table 2.
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Copyright: © 2026 Al-Saadi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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