Purification and bioactivity characterization of LT subunit proteins (IMAGE)

Institute of Microbiology, Chinese Academy of Sciences

Purification and bioactivity characterization of LT subunit proteins

Caption

 A: SDS-PAGE analysis of purified LTA, LTA(R192G), and LTB proteins [Lane M: Protein molecular weight marker; Lanes 1, 2: LTA protein; Lanes 3, 4: LTA(R192G) protein; Lanes 5, 6: LTB protein]; B: Native-PAGE analysis of LTB protein (Lane 1: Protein under non-denaturing conditions; Lane 2: Protein under heat-denaturing conditions); C: ELISA results for the binding of LTB protein to the GM1 receptor; D: Changes in intracellular cAMP levels after treatment of cells with LTA, LTA(R192G), and LTB proteins; E: Statistical analysis of mean intracellular fluorescence intensity; F: Cellular uptake of fluorescently labeled LTA, LTA(R192G), and LTB proteins (magnification: 200×).

Credit

Acta Microbiologica Sinica

Usage Restrictions

News organizations may use or redistribute this image, with proper attribution, as part of news coverage of this paper only.

License

Original content

Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.