Figure 6: Metabolic profile of healthy donor or multiple myeloma patient's memory T cell population varies depending upon the peptide cocktail used for stimulation. (IMAGE)

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Figure 6: Metabolic profile of healthy donor or multiple myeloma patient's memory T cell population varies depending upon the peptide cocktail used for stimulation.

Caption

Glycolysis stress test was conducted to examine the extracellular acidification rate (ECAR) in response to glucose, oligomycin, and 2-deoxy-D-glucose (2DG). The Mito-cell stress test assessed the oxygen consumption rate (OCR) following treatment with oligomycin, carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP) and rotenone/antimycin. The histograms for ECAR (left panel) and OCR (right panel) are depicted for T cells generated following exposure of PBMCs from healthy donor (blue histogram) or multiple myeloma patient (red histogram) to MUC1 cocktail (A), cocktail 1 (B), cocktail 3 (C) and cocktail 4 (D). Representative data are shown and no statistically significant differences were observed among the six samples analyzed (Student's t-test).

Credit

Correspondence to - Sandra J. Gendler - gendler.sandra@mayo.edu

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