High resolution imaging reveal an increased migration speed of the epithelial cell line MCF7 clones upon CRISPR/dCas IGFBP2 epigenetic editing. (IMAGE)
Caption
(A) Display of 9 independent migratory tracks from 3 pooled control sgRNAs 1-3 clones and 3 pooled IGFBP2 sgRNAs 3 and 6 clones. (B) Distribution of migration velocity from 3 pooled control sgRNAs 1-3 clones and 3 pooled IGFBP2 sgRNAs 3 and 6 clones. Error bars represent the SD of n = 3–9 independent positions. Statistical analysis were performed using Mann-Whitney U test for speed and displacement and T-test for mitotic cell division analysis. p values ≤ 0.05, ≤ 0.01, or ≤ 0.001 are considered statistically significant and indicated by an asterisk (*, **, or ***, respectively). (C) Representative time-lapse images of 3 pooled control sgRNAs 1-3 clones and 3 pooled IGFBP2 sgRNAs 3 and 6 clones. White arrow indicates the appearance of cell protrusions (absent in the control conditions). Images show nuclear marker (H2B-dendra2) to follow cell divisions and migration and membrane markers (mTurquoise-2 and mVenus) to study cell morphology.
Credit
Correspondence to - Miguel Vizoso - orcid.org/0000-0002-9992-2851 and Jacco van Rheenen - j.v.rheenen@nki.nl
Usage Restrictions
Credit must be given to the creator.
License
CC BY