[Figure 1] Distinct role of the interactions of RPA32 and RPA70 with XPA in NER (IMAGE)

Institute for Basic Science

[Figure 1] Distinct role of the interactions of RPA32 and RPA70 with XPA in NER

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Cells were irradiated with UV and the colocalization of XPA with UV damage sites was visualized by fluorescence microscopy. DNA damage is highlighted by red arrows and co-localization of XPA with UV DNA is indicated by yellow arrows. In XPA-RPA32 mutant cells (M32-4), reduced XPA recruitment to DNA damage was observed. In XPA-RPA70 mutant cells (M70-6), XPA remained bound at damaged sites for an extended time. This indicates that RPA32 interaction with XPA is required for recruitment of XPA to UV-induced damage, while RPA70 interaction with XPA is important for positioning of XPA for completion of NER.

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Institute for Basic Science

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