According to the target classification level to select appropriate primers and databases for soil nematodes (IMAGE)
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NMDS indicated that there were significant differences in the nematode communities obtained by different primers and different database annotations (A and B). Compared with the primer NF1F, higher values were found in indices annotated by the primer 3NDF, especially for evenness index. For the primer 3NDF, the Chao index obtained by the three databases were slightly different, with higher values observed in the NT database (C and D)
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