Genome editing medaka (IMAGE)

Heidelberg University

Genome editing medaka

Caption

Following successful genome editing of the oca2 gene, the ratio of originally pigmented to unpigmented cells in the embryonic eye of the Japanese ricefish medaka serves as readout for Cas9 efficiency. This was used to optimise the CRISPR/Cas9 system. Low knock-out efficiency of standard Cas9 enzymes (top) and increased knock-out rate using the improved heiCas9 (bottom).

Credit

Thomas Thumberger (COS)

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