O-ClickFC for high-throughput analysis of single-cell lipid metabolism at the organelle level (IMAGE)
Caption
O-ClickFC is applicable to CRISPR screening for discovery of genetic factors underlying human lipid metabolism. Target lipids in live cells are fluorescently labeled via spatially limited click reactions using different-colored organelle-targeting reagents. This allows for flow cytometric high-throughput analysis of their lipid contents and distribution at the organelle level on the basis of fluorescent intensity and spectrum of labeled lipids. Fluorescent patterns on labeled lipids in individual cells reflect the metabolic status such as defects in lipid biosynthesis or trafficking caused by CRISPR gene editing, thereby enabling large-scale genetic screening based on subcellular lipid phenotypes.
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JST
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Non
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