Structural analysis and deep mutational scanning (DMS) of AsCas12f (IMAGE)
Caption
The team used cryogenic electron microscopy, a method to look at the structure of biological molecules in high-resolution, to analyze AsCas12f and engineer their new version. The DMS “heatmap” illustrates how all single mutations affected genome-editing activity. Blue squares indicate an undesirable mutation, while red ones represent desirable changes. The darker the color, the greater the effect.
Credit
Hino et al. 2023
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License
CC BY-ND