Figure 2 | STORM fusion using the rFRC map. (IMAGE)
Caption
(a) Schematic of the STORM fusion. 'ME': Multi-emitter MLE result; 'SE': single-emitter Gaussian fitting result. (b) STORM results (COS-7 cells, α-tubulin labeled with Alexa Fluor 647, left) and their rFRC maps (right) are shown from top to bottom, which are magnified views of the white box in (d). From top to bottom: 'ME' result; 'SE' result; the fused result from the 'ME' and 'SE' reconstructions. The corresponding rFRC values are marked on the top left of the rFRC maps. (c) Magnified views of the dashed circles in (b). From left to right: ME results, SE results, fusion weights (inverted rFRC maps of ME results and SE results merged as green and magenta channels, respectively), and fused STORM results. (d) The entire view of the fused STORM result (COS-7 cells, α-tubulin labeled with Alexa Fluor 647). (e) rFRC map of (d). The inset shows the improved resolution achieved by fusion compared with the SE (80.55 ± 1.52 nm at 22.0% region, hollow) and ME (4.28 ± 0.14 nm at 19.2% region, white solid) results. (f) Enlarged regions enclosed by the yellow box in (d). The results of the rFRC map, fused STORM, and RSM are shown from top to bottom. scale bars: (b, c) 500 nm; (d) 5 μm; (f) 1 μm.
Credit
by Weisong Zhao, Xiaoshuai Huang, Jianyu Yang, Liying Qu, Guohua Qiu, Yue Zhao, Xinwei Wang, Deer Su, Xumin Ding, Heng Mao, Yaming Jiu, Ying Hu, Jiubin Tan, Shiqun Zhao, Leiting Pan, Liangyi Chen & Haoyu Li
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License
CC BY