Imaging neural activity in singing flies (VIDEO)
Caption
Using a combination recording device and microscope, researchers can image the neural activity in the fly’s nerve cord while the insect sings. This video shows an example trial of calcium imaging of TN1A neurons. Top left: a bottom view of the fly. The left side of the image corresponds to the right side of the fly. Bottom left: the microphone signal for the right wing. Top right: raw fluorescent signals averaged across z-planes. The left side of the image corresponds to the left side of the ventral nerve cord. Bottom right: schematic of the imaged volume. Red boxes represent the timing of optogenetic stimulation. Calcium signals of individual TN1A neurons increased in response to optogenetic stimulation of the song driver.
Credit
Shiozaki et al. DOI: 10.1101/2022.12.14.520499
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