Effects of peptides on cell membrane integrity. (IMAGE)
Caption
Laser confocal scanning microscopy images of Staphylococcus aureus ATCC 6538 after treatment with 2 μM SFK2 for 2 h. Scale bar: 5 μm. B. Laser confocal scanning microscope images of S. aureus ATCC 6538 and E. coli ATCC 25922 after treatment with 2 μM SFK2 for 2 h. Scale bar: 5 μm. C. Flow cytometry analysis of membrane integrity of S. aureus ATCC 6538, Pseudomonas aeruginosa ATCC 27853, and E. coli ATCC 25922 treated with different concentrations of SFK2. D. SEM images of S. aureus ATCC 6538 treated with 2 μM SFK2 for 2 h. Scale bar of SEM: 2 μm. E. TEM images of S. aureus ATCC 6538 treated with 2 μM SFK2 for 2 h. Scale bar of TEM: 500 nm. PI, Propidium Iodide; P2, proportion of cells generating PI fluorescent signals; SEM, scanning electron microscope; TEM, transmission electron microscopy.
Credit
Xi Ma, State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University
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