Nano-XPCT analysis of the Paneth and goblet cells (IMAGE)
Caption
Representative 3d rendering of the longitudinal view of one crypt of SAMR1 mouse. the epithelial layer
of the crypt has been rendered in green. the Paneth cells are colored in yellow and the goblet cells in blue. Scale bars, 5 μm. (B) and (C) show 3d renderings and nano-XPct
close-ups of goblet and Paneth cells, respectively. the same color code of (A) has been used for the 3d renderings shown in (B) and (c). in detail, the goblet cell nucleus is
colored in dark blue and the apical portion, expanded with mucin-secreting granules, which extends into the intestinal lumen, is rendered in light blue. the Paneth cell
presents the typical pyramidal shape with basally situated nucleus (blue) and prominent apical granules (yellow) that occupy most of its cytoplasmatic region. nano-XPct
close-ups in (B) show goblet cells (indicated by arrows) secreting mucus (asterisks) in the intestinal lumen. Scale bars, 2.5 μm. in (c), transversal view of a crypt in which
the Paneth cells are arranged in a radial pattern (highlighted by the dashed-line boxes). the release of the antimicrobial granules (black dots, asterisk) into the lumen is
visible. Scale bars, 5 μm. (D and E) Quantification of goblet and Paneth cells in the crypts. Results are obtained on 30 crypt per mouse (n = 1) and are shown as
mean ± Sd. One-way AnOvA P < 0.0001; post hoc by tukey’s post hoc test: *P < 0.02, **P < 0.01, ***P < 0.001, and ****P < 0.0001
Credit
Palermo, F. et al, Science Advances, 31 January 2025
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