Butyrate treatment limits inflammatory induction by intestinal faeces. (IMAGE)
Caption
Small intestinal faecal contents were collected from SV (n=5) or (n=6) mice 1 day after combined injury. Faecal contents were homogenised in PBS to generate faecal slurry. MODE-K cells were treated with 2 mM sodium butyrate in PBS or PBS alone; 1 hour later, small intestinal faecal slurry was added for 24 hours and then conditioned media was collected for protein quantification of IL-6 via ELISA and normalised to conditioned media with no treatment. Graphs depict the average result from the faecal slurry of individual mice as a single point in addition to group means±SEM. Significance was analysed by one-way ANOVA with Tukey’s multiple comparisons *p<0.05, **p<0.01, ***p<0.001. (B, C) Paired t-test of MODE-K conditioned media with or without 2 mM sodium butyrate and addition of (B) SV faeces slurry n=5, or (C) EB faeces slurry n=6. Significance was analysed by paired t-test. *p<0.05, ****p<0.0001. ANOVA, analysis of variance; EB, ethanol burn; IL-6, interleukin-6; MODE-K, murine duodenal cell clone-K; SV, sham vehicle.
Credit
By Caroline J Herrnreiter, Mary Grace Murray, Marisa Luck, et al.
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CC BY-NC