NR2F1 directly binds to STAT3 and regulates the expression of p-STAT3. (IMAGE)
Caption
The protein levels and quantification of JAK1, p-STAT3, SMAD2, and MYD88 in TGF-β1-treated SRA01/04 cells with or without Sh-NR2F1. n = 3 per group; mean ± standard deviation; ns, >0.05; **P < 0.01; unpaired student's t-test. (C, D) The protein level and quantitative chart of p-STAT3 in AAV-NC or AAV-NR2F1 ASC mice. n = 3 per group; mean ± sstandard deviation; *P < 0.01; unpaired student's t-test. (E) The motif of NR2F1 predicted by the JASPAR website. (F) A dual-luciferase vector. (G) Mutant and wild-type STAT3 plasmids consequences. (H) The dual-luciferase assay for NR2F1 and STAT3 promoter. n = 3 per group; mean ± standard deviation; ns, >0.05; *P < 0.05; unpaired student's t-test. (I, J) The protein level of p-STAT3 in TGF-β1-induced SRA01/04 cells following NR2F1 agonist treatment at 0.5 or 1 μM. n = 3 per group; mean ± standard deviation; ns >0.05; *P < 0.05; one-way ANOVA. (K) Structure of p-STAT3 specific inhibitor NSC 74859. (L, M) The protein expression and quantification of p-STAT3, STAT3, JAK1, FN1, VIM, and α-SMA in TGF-β1-induced SRA01/04 cells treated with the specific P-STAT3 inhibitor. n = 3 per group; mean ± standard deviation; ns, >0.05, *P < 0.05, **P < 0.01; one-way ANOVA. (N, O) The protein expression of the apoptosis-related markers BAX and CASP3 in TGF-β1-induced SRA01/04 cells treated with NSC 74859. n = 3 per group; mean ± standard deviation; *P < 0.05; one-way ANOVA. NR2F1, nuclear receptor subfamily 2 group F member 1; STAT3, signal transducer and activator of transcription 3; p-STAT3, phosphorylated STAT3; ASC, anterior subcapsular cataract; FN1, fibronectin 1; α-SMA, α-smooth muscle actin; VIM, vimentin; BAX, Bcl-2-associated X; CASP3, caspase 3; TGF-β1, transforming growth factor-β1; JAK1, Janus kinase 1; SMAD2, SMAD family member 2; AAV, adeno-associated adenovirus.
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Genes & Diseases
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