KLF15 enriches at Jmjd1c-SE and promotes the expression of Jmjd1c. (IMAGE)
Caption
(A) The binding site motif of transcription factor KLF15. (B) Quantitative PCR was performed to detect the overexpression efficiency of KLF15 in RN-sc cells. (C) Immunofluorescence was used to detect the protein level of Jmjd1c upon overexpression of KLF15. (D) Quantitative PCR was used to detect the knockdown efficiency of KLF15 in RN-sc cells. (E) Immunofluorescence detection showed the expression of Jmjd1c after knocking down KLF15 expression. (F) Genome browser tracks showed three potential binding regions of KLF15 and Jmjd1c. (G) RN-sc cells were co-transfected with three potential KLF15-specific elements (E1, E2, and E3) and Jmjd1c, and luciferase activity was determined using a dual-luciferase assay system. (H) Dual-luciferase assay showed the luciferase activity changes of KLF15-specific elements (E2 and E3) in RN-sc cells after knocking down KLF15. (I) Chromatin immunoprecipitation‐quantitative PCR was performed on RN-sc and SNI rats to evaluate the binding ability of KLF15 to E1, E2, and E3 elements of Jmjd1c-SE. The statistical results were presented as mean ± standard deviation. Student's t-test was utilized to evaluate the differences between the two groups. The asterisks indicate significance values (∗∗∗P < 0.001).
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Genes & Diseases
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