Effects of SIRT5 and/or BMP9 on the protein level of HIF-1α in MEFs. (IMAGE)

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Effects of SIRT5 and/or BMP9 on the protein level of HIF-1α in MEFs.

Caption

(A) Western blot assay shows the impact of BMP9 and/or SIRT5 on the protein level of HIF-1α in MEFs. (B) Quantitative analysis of western blot assay shows the impact of BMP9 and/or SIRT5 on the protein level of HIF-1α in MEFs. (C) Western blot assay shows the impact of SIRT5 knockdown on the protein level of HIF-1α in MEFs. (D) Quantitative analysis of western blot assay shows the impact of SIRT5 knockdown on the protein level of HIF-1α in MEFs. (E) Western blot assay shows the impact of BMP9 and/or SIRT5 on the protein level of HIF-1α in MEFs. (F) Quantitative analysis of western blot assay shows the impact of BMP9 and/or SIRT5 on the protein level of HIF-1α in MEFs. (G) Western blot assay shows the impact of BMP9 and/or SIRT5 knockdown on the protein level of HIF-1α in MEFs. (H) Quantitative analysis of western blot assay shows the impact of BMP9 and/or SIRT5 knockdown on the protein level of HIF-1α in MEFs. (I) Immunofluorescent staining and confocal assay show the impact of BMP9 and/or SIRT5 knockdown on the protein level of HIF-1α in MEFs. (Scale bar = 40 μm “∗” P<0.05, “∗∗” P<0.01 vs control. BMP9, bone morphogenetic protein 9; SIRT5, sirtuin 5; MEFs, mouse embryonic fibroblasts; HIF-1α, hypoxia-inducible factor 1 subunit alpha.)

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Genes & Diseases

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