Figure 2. Tissue-clearing techniques and three-dimensional fluorescence imaging (IMAGE)

Hiroshima University

Figure 2. Tissue-clearing techniques and three-dimensional fluorescence imaging

Caption

Steps for deep-tissue 3D-imaging. Embryos or neonates fixed with 4% PFA were optically cleared with 60% TDE or tissue-clearing reagent CUBIC-L/R, and imaged using stereoscopic, confocal, or two-photon microscopy.

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Reproduced with permission from Development, Yu et al., 2025.

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The Company of Biologists, used with permission for press release purposes only.

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