The MEN1 gene is identified as an oncogene in BLCA. (IMAGE)
Caption
(A) The expression status of the MEN1 gene in BLCA datasets based on the TCGA database. (B) The correlation between the MEN1 gene and tumor stages in BLCA tissues based on the TCGA database. (C) The survival analysis indicates that the MEN1 gene is a risk factor and is related to the survival and prognosis of BLCA tissues based on the GEO database (GSE31684 and GSE13507). (D) Representative immunohistochemistry staining images are presented for immunostaining of menin in different stages of BLCA tissues. Scale bar, 100 μm. (E) Enrichment analysis of the high expression of the MEN1 gene of BLCA patients based on GSEA 4.2.3 software (https://www.gsea-msigdb.org/gsea/index.jsp). (F) The correlation between the MEN1 gene and cell cycle checkpoint-related markers in BLCA based on the TCGA database. (G, H) The quantitative real-time PCR and western blotting assays analyzed the expression level of the MEN1 gene and menin protein in human immortalized uroepithelial cell line (SV-HUC-1) and BLCA cell lines (T24, 5637, and HT-1197), respectively. (I) Images of representative nude mouse xenograft model studies. T24 tumor xenografts excised from male BALB/c (nu/nu) nude mice after 28 days of treatments with the in vivo RNA interfering of MEN1 knockdown (MEN1-KD group) or control. (J) Days versus tumor volume curves for T24 tumor xenografts showed that MEN1-KD inhibited tumor growth compared with the control group. (K) Representative immunohistochemistry staining images of menin, Ki67, CDK2, and CDK4 were presented in MEN1-KD-treated or control-treated T24 xenograft nude mice tissues. Scale bar, 50 μm. ns, non-significant; *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.001 versus control. MEN1, multiple endocrine neoplasia type 1; BLCA, bladder cancer; CDK2/4, cyclin-dependent kinase 2/4.
Credit
Qing Shi, Xiang Pan, Shiheng Zhang, Mengyuan Wu, Meiqi Xu, Yun-Qi Li, Li Zhong, Zi-Qi Wang, Wanhai Xu, Yakun Luo
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