TFAP2C is crucial for the regulation of CTNNB1 by menin in BLCA cells (IMAGE)
Caption
(A, B) mRNA and protein expression of TFAP2C, CTNNB1, CDK2, CDK4, CCND1, CCNE1, and MYC were analyzed by RT-qPCR and western blotting in TFAP2C-KD T24, 5637, and HT-1197 cells. (C) Schematic representation of the position of ChIP-qPCR primers along the CTNNB1 promoter. (D) The levels of TFAP2C recruitment to the CTNNB1 promoter in siCtrl or siTFAP2C-treated T24, 5637, and HT-1197 cells, detected by ChIP-qPCR. (E) RT-qPCR analysis showed the expression of MEN1, TFAP2C, and CTNNB1 in OE-menin-treated, siTFAP2C-treated, or OE-menin plus siTFAP2C-treated BLCA cells. (F) The levels of menin recruitment to the CTNNB1 promoter in OE-menin-treated, or OE-menin plus siTFAP2C-treated T24, 5637, and HT-1197 cells, detected by ChIP-qPCR. ns, non-significant; *P < 0.05, **P < 0.01, and ***P < 0.001 versus control. TFAP2C, transcription factor AP-2 gamma; BLCA, bladder cancer; RT-qPCR, quantitative real-time PCR; MEN1, multiple endocrine neoplasia type 1; CTNNB1, catenin beta 1; ChIP, chromatin immunoprecipitation; CDK2/4, cyclin dependent kinase 2/4; CCND1, cyclin D1; CCNE1, cyclin E1.
Credit
Qing Shi, Xiang Pan, Shiheng Zhang, Mengyuan Wu, Meiqi Xu, Yun-Qi Li, Li Zhong, Zi-Qi Wang, Wanhai Xu, Yakun Luo
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted.
License
CC BY-NC-ND