Highly purified fibroblasts were successfully obtained from human CRC tissues. (IMAGE)

Sichuan International Medical Exchange and Promotion Association

Highly purified fibroblasts were successfully obtained from human CRC tissues.

Caption

(A) Process of cancer‐associated fibroblasts (CAFs) and normal fibroblasts (NFs) isolation from human colorectal cancer (CRC) tissues and normal adjacent tissues (NATs) via enzymatic digestion. (B) Representative morphology images of CAFs and NFs. Scale bar, 100 μm. (C, D) Representative immunofluorescence images of biomarkers in CAFs and NFs. Merged images represent overlays of DAPI (blue) with vimentin (red), cytokeratin AE1–AE3 (red) and smooth muscle actin alpha (α‐SMA) (green). DAPI: nuclear marker. Scale bar, 50 μm. (E, F) The mRNA levels of actin alpha 2 (ACTA2, a gene encoding the α‐SMA protein) and the protein levels of α‐SMA in CAFs and NFs. The data are presented as the mean ± SD. *p < 0.05, ***p < 0.001; Student's t‐test.

Credit

Yinglan Zhao & Xiao Du

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