mRNAs converge (IMAGE)
Caption
This large, stitched composite created from many high-magnification images shows four mRNAs localized in the neuropil, the dense mesh of synaptic connections in a layer distinct from the cell bodies, in the juvenile mouse hippocampus. mRNAs were labeled using the HiPlex RNAscope in situ hybridization assay to detect Adcy1 (green), Aco2 (magenta), Psd (cyan), and Dlg4 (yellow), and nuclei were counterstained with DAPI (blue). This technique was used to illustrate how these mRNAs naturally appear in close proximity simply because they are abundant.
Credit
Shannon Farris/Virginia Tech
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